Journal: Biomaterials Research

Article Title: In Silico Target Discovery of Kaempferol: Therapeutic Effect of Kaempferol on Atopic Dermatitis through Regulation of Aryl Hydrocarbon Receptor

doi: 10.34133/bmr.0270

Figure Lengend Snippet: The 3-dimensional (3D) docking pose and 2-dimensional (2D) interaction diagrams of KF with potential targets. (A to E) Interactions between KF and associated residues in the interface of arachidonate 5-lipoxygenase (ALOX5; Protein Data Bank [PDB] ID: 3O8Y), carbonic anhydrase 2 (CA2; PDB ID: 1A42), carbonic anhydrase 12 (CA12; PDB ID: 1JCZ), ATP binding cassette subfamily B member 1 (ABCB1; PDB ID: 7OTI), and AhR proteins (PDB ID: 7VNA) were generated using Discovery Studio (v21.1, Dassault Systèmes, San Diego, CA, USA). (E) KF interactions with AhR interface residues (PDB ID: 7VNA). (F) HaCaT cells were seeded at 5 × 10 3 cells/ml, cultured for 24 h, and then treated with the indicated concentrations of KF (20 and 40 μM), and then the messenger RNA (mRNA) levels of the indicated genes were evaluated using real-time quantitative polymerase chain reaction (qPCR). The results represent the mean ± SD of 3 independent experiments ( n = 3). * P < 0.05 versus the control group (KF 0 μM).

Article Snippet: The 2D interaction graphics and 3D docking poses were displayed using BIOVIA Discovery Studio v21.1 (Dassault Systèmes, San Diego, CA, USA).

Techniques: Binding Assay, Generated, Cell Culture, Real-time Polymerase Chain Reaction, Control

Journal: Smart Medicine

Article Title: Pathogen‐binding nanoparticles to inhibit host cell infection by heparan sulfate and sialic acid dependent viruses and protozoan parasites

doi: 10.1002/SMMD.20230046

Figure Lengend Snippet: Efficacy comparison of selected antiviral NPs based on HS/SA mimetic structures, including a few historic examples but mostly recent formulations where EC 50 /IC 50 values or curves were available.

Article Snippet: The same 2D graphene platform was also effective against IAV by substituting the sulfates with SA moieties.

Techniques: Comparison, Virus, Animal Model

Journal: Smart Medicine

Article Title: Pathogen‐binding nanoparticles to inhibit host cell infection by heparan sulfate and sialic acid dependent viruses and protozoan parasites

doi: 10.1002/SMMD.20230046

Figure Lengend Snippet: Non‐spherical nanodesigns for virus inhibition. (A) Schematic of heparin/HS modified T1 DNA origami shells and corresponding electron micrographs showing successful caging of various virus types (Scale bar, 100 nm). Modified and reprinted under terms of the CC‐BY license. ¹⁰⁶ Copyright 2022, The Authors, published by American Chemical Society. (B) 2D graphene nanosheets with sulfated dendritic groups and hydrophobic chains (≥10 carbons in length) achieved potent SARS‐CoV‐2 inhibition with a virucidal mechanism (bottom images show plaque assay). *** p < 0.001. Modified and reprinted under terms of the CC‐BY license. ⁵⁷ Copyright 2021, The Authors, published by John Wiley and Sons. HS, heparan sulfate.

Article Snippet: The same 2D graphene platform was also effective against IAV by substituting the sulfates with SA moieties.

Techniques: Virus, Inhibition, Modification, Plaque Assay